System and method for plasma reduced platelet collection

ABSTRACT

A method and apparatus for collecting plasma reduced platelets potentially suspended in a synthetic solution from a donor. Whole blood is drawn from the donor and introduced into a separation chamber. Platelets are extracted from the separation chamber into a container, using, for example, surge (with anticoagulated plasma or a synthetic solution) or push methodologies. The remaining blood components in the separation chamber are returned back to the donor. The steps of drawing whole blood and introducing the whole blood into the separation chamber, extracting platelets from the separation chamber into the container, and returning the remaining components in the chamber back to the donor are repeated. The sequestered platelets in the container are reintroduced into the separation chamber, whereupon a plasma reduced platelet product is extracted.

PRIORITY

This application is a divisional of co-pending U.S. patent application Ser. No. 12/102,486, entitled “System and Method for Plasma Reduced Platelet Collection,” filed Apr. 14, 2008, and naming Toshiyasu Ohashi, Etienne Pagès, Dominique Uhlmann, Pascal Maillard, and Michael Ragusa as inventors, the disclosure of which is incorporated herein, in its entirety by reference.

TECHNICAL FIELD

The present invention relates to systems and methods for platelet collection, and particularly to systems and methods for concentrating and collecting platelets.

BACKGROUND ART

Apheresis is a procedure in which individual blood components can be separated and collected from whole blood temporarily withdrawn from a subject. Typically, whole blood is withdrawn through a needle inserted into a vein of the subjects arm and into a cell separator, such as a centrifugal bowl. Once the whole blood is separated into its various components, one or more of the components can be removed from the centrifugal bowl. The remaining components can be returned to the subject along with optional compensation fluid to make up for the volume of the removed component. The process of drawing and returning continues until the quantity of the desired component has been collected, at which point the process is stopped. A central feature of apheresis systems is that the processed but unwanted components are returned to the donor. Blood components separated may include, for example, a high density component such as red blood cells, an intermediate density component such as platelets or white blood cells, and a lower density component such as plasma.

Among various blood component products obtainable through apheresis, the demand for plasma reduced platelet products is rapidly growing. This is particularly because, with the improvement in cancer therapy, there is a need to administrate more and more platelets to patients with lowered hemopoietic function. Platelets are fragments of a large cell located in the marrow called a megakaryocyte and primarily contribute to hemostasis by performing aggregation function, although they also have a role in tissue healing. Normal platelet counts are 150,000-400,000/mm³ in the adult. Platelet counts under 20,000/mm³ can cause various troubles such as spontaneous bleeding.

Platelets have a short half-life of 4-6 days and the number of donors is limited. Therefore, in producing plasma reduced platelet products, it is important to harvest platelets from the whole blood supplied by a donor at a maximum yield and in a required amount. Further, it is known that the contamination of plasma reduced platelet product by white blood cells can lead to serious medial complications, such as graft versus host (“GVH”) reactions. Therefore, it is also very important to keep the level of contamination by white blood cells as low as possible, while efficiently collecting platelets. To this end, various techniques have been developed. For example, using “surge” technology, after whole blood is collected and concentrically separated within a centrifuge into higher density, intermediate density and lower density components and plasma is harvested (so-called “draw” step), the plasma is supplied through the centrifuge at a surge flow rate, that is, a flow rate that increases with time. By performing the surge, platelets can be preferentially displaced from the intermediate density components, which exist as a buffy coat mainly comprising a mixture of platelets and white blood cells, and plasma reduced platelet products can thereby be produced at an increased yield. Instead of using surge technology, the platelet layer can also be extracted from the centrifuge by means of a layer “push” in which anticoagulated whole blood is introduced into the bowl until the platelet layer is pushed out, or by using a combination of surge and push methodologies. After harvesting a desired component or components, the residual blood components mostly comprising red blood cells are returned to the donor (so-called “return” step).

Typically, 450-500 ml of whole blood is processed during one cycle which comprises the above-mentioned successive steps. This amount is based on 15% or less of the total amount of blood in humans and, if more than this amount is taken out of the body at once, the donor may suffer from blood pressure lowering or dizziness. Using surge technology, the concentration of the sequestered platelet product ranges from 0.8×10⁶/μL to 2.6×10⁶/μL (typically 1.5×10⁶/μL), with moderate leukocyte concentration. Pushed platelet product concentration tends to be higher but leads to greater leukocyte and red blood cell residual contamination.

This resulting platelet concentration is often too low for platelet product compatibility with arising pathogen inactivation methods. Additionally, simultaneous plasma collection of one to two additional plasma units may be prevented due to the relatively high volume of plasma captured with the platelet product. The relatively high plasma protein content in the platelet product is also less desirable in terms of recipient tolerance.

SUMMARY OF THE INVENTION

In accordance with one embodiment of the invention, a method for collecting plasma reduced platelets from a donor is presented. Whole blood is drawn from the donor, anticoagulated, and introduced into a separation chamber. Platelets are extracted from the separation chamber into a container and the remaining blood components in or out of the separation chamber are returned back to the donor. The steps of drawing whole blood and introducing the whole blood into the separation chamber, extracting platelets from the separation chamber into the container, and returning the remaining components back to the donor are repeated. After a desired quantity of platelets is sequestered into the container, platelets from the container are reintroduced into the separation chamber, whereupon a plasma reduced platelet product is extracted.

In accordance with related embodiments of the invention, extracting platelets and/or the plasma reduced platelet product from the separation chamber may include extracting platelets by surge elutriation or layer push methodologies or a combination of both. Whole anticoagulated blood may be added to the separation chamber prior to reintroducing platelets from the container into the separation chamber, so as to form a cell bed at a periphery of the separation chamber. Additionally or alternatively, whole anticoagulated blood may be added to the separation chamber during platelet reintroduction so as to bring platelet layer towards the elutriation radius, or after platelet reintroduction for perfecting platelet separation and standardizing conditions of initiating platelet extraction. Returning remaining blood components in the separation chamber back to the donor may include returning back to the donor plasma and/or red blood cells.

In further related embodiments of the invention, the steps of drawing whole blood and introducing the whole blood into the separation chamber, extracting platelets from the separation chamber into the container, returning the remaining components in the chamber back to the donor may be repeated until a desired volume of platelets is extracted. In some embodiments, reintroducing the platelets from the container and extracting a plasma reduced platelet product is only done once. However, in other embodiments, the steps may be repeated until a desired volume or concentration of reduced plasma platelet product is obtained. Additionally or alternatively, plasma may be added to the plasma reduced platelet product to adjust the plasma reduced platelet product to a pre-determined volume or predetermined concentration. This plasma can be added through the bowl or via a dedicated plasma line.

In accordance with another embodiment of the invention, a system for collecting plasma reduced platelets from a donor includes means for drawing whole blood from the donor. A separation chamber separates the whole blood into a plurality of components, the components including a platelet component. The platelet component is stored in a container. The system also includes a means for returning at least one of the plurality of components from the bowl back to the to the donor, and a flow means for connecting the means for drawing whole blood, the separation chamber, the container, and the means for returning at least one of the plurality of components. A controller controls the flow means, the means for returning, and the separation chamber so as to repeatedly draw whole blood from the donor into the separation chamber, extract platelets from the separation chamber into the container, and return remaining components in the separation chamber back to the donor. After a predetermined volume of platelets has been sequestered in the container, platelets from the container are reintroduced into the separation chamber so as to extract a plasma reduced platelet product from the separation chamber.

In accordance with yet another embodiment of the invention, a system for collecting plasma reduced platelets from a donor is presented. An apheresis system draws whole blood from a donor and separates the whole blood into a plurality of components including a platelet component and a non-platelet component using a separation chamber. The platelet component is stored into a container while the non-platelet blood components are returned back to the donor. A controller controls the apheresis system such that upon obtaining a predetermined volume of platelets in the container, platelets from the container are reintroduced into the separation chamber so as to extract a plasma reduced platelet product from the separation chamber.

In accordance with another embodiment, a method for plasma reduced blood component collection during blood processing is presented. The method draws blood from a subject through a venous-access device and into a blood component separation device until a predetermined amount of withdrawn blood is in the blood component separation device. The drawn blood is then centrifuged within the blood component separation device such that the withdrawn blood is separated into at least a first blood component and a second blood component. The method then removes the first blood component from the blood component separation device using a surge elutriation method such that the first blood component is transferred to a first component storage container. The method then returns the second blood component to the subject through the venous-access device. The draw and return steps may be repeated one or more times until an appropriate amount of first blood component is removed.

The method then partially fills the blood component separation device with whole blood and reintroduces the removed first blood component into the blood component separation device. Reintroducing the first blood component creates an enlarged layer of the first blood component within the blood component separation device. The enlarged layer of first blood component can be removed from the blood component separation device using a surge elutriation method such that the enlarged layer of first blood component is transferred to the first blood component storage container. The remaining blood components, including the second blood component can be returned to the subject through the venous-access device.

In some embodiments, centrifuging the blood further separates the blood into a third blood component in addition to the first blood component and the second blood component. The first blood component may be platelets, the second blood component may be red blood cells, and the third blood component may be plasma. Additionally, the surge elutriation method may include reintroducing removed plasma (e.g., the third blood component) into the blood component separation device at an increasing rate until the first blood component (e.g., the platelets) is removed from the blood component separation device. Returning the second blood component to the subject may also include returning the plasma reintroduced into the blood component separation device (during the surge process) to the subject.

In still other embodiments, a system for plasma reduced blood component collection during blood processing comprising is presented. The system may include a venous access device for drawing a first volume of whole blood from a subject and returning blood components to the subject. The system may also include a blood component separation device, a return line, and a reintroduction line. The blood component separation device separates the drawn blood into a first blood component and a second blood component. The blood component separation device may also be configured to send the first blood component to a first blood component bag using a surge elutriation method. The return line fluidly connects the venous-access device and the blood component separation device and is used to return the second blood component to the subject. The reintroduction line fluidly connects the first blood component bag and the blood component separation device, and is used to reintroduce the first blood component into the blood component separation device when a second volume of whole blood is withdrawn from the subject. Reintroducing the first blood component creates an enlarged layer of the first blood component within the blood component separation device. The enlarged layer of first blood component may be removed from the blood component separation device using a surge elutriation method.

BRIEF DESCRIPTION OF THE DRAWINGS

The foregoing features of the invention will be more readily understood by reference to the following detailed description, taken with reference to the accompanying drawings, in which:

FIG. 1 is a schematic diagram of an apheresis machine, in accordance with one embodiment of the invention;

FIG. 2 is a schematic diagram of a disposable system for use with the machine of FIG. 1, in accordance with one embodiment of the invention;

FIG. 3 is a side view of a centrifuge bowl for use with the machine of FIG. 1, in accordance with one embodiment of the invention;

FIG. 4 is a flow chart depicting a method for collecting plasma reduced platelets from a donor, in accordance with one embodiment of the invention; and

FIG. 5 is a schematic diagram of a three-line apheresis machine, in accordance with additional embodiments of the invention.

DETAILED DESCRIPTION OF SPECIFIC EMBODIMENTS

Referring to FIGS. 1 and 2, an apheresis apparatus 10 uses a blood component separation device, such as a standard Latham type centrifuge 11 for separating anticoagulated whole blood into its constituent components, as described in U.S. Pat. No. 3,145,713, which is hereby incorporated by reference. Other types of separation chambers and devices may be used, such as, without limitation, an integral blow-molded centrifuge bowl, as described in U.S. Pat. Nos. 4,983,156 and 4,943,273, which are hereby incorporated by reference. The centrifuge 11 includes a rotating bowl 12 and stationary input and output ports PT1 and PT2 that are typically closely coupled to the bowl interior by a rotary seal 74 (see FIG. 3). The input port PT1 of the centrifuge 11 is in fluid communication with a venous access devices 24 (e.g., a phlebotomy needle) via a blood filter F1, a tube 28 and a Y-connector 30 when a valve V1 is open. The venous access device 24 may be replaced with a whole blood bag (not shown) in case the whole blood is to be first pooled and then supplied. The tube 28 has compatibility with blood, as is all the tubing in the apparatus 10. The outlet port PT2 of the centrifuge 11 is selectively coupled by a tube 36, a valve V2 and a tube 37 with a first container 18 labeled plasma suspended from a weight scale 33. A second container 20 labeled platelets is selectively coupled via the tube 36, a valve V3 and a tube 39 to the outlet port PT2. Additionally, a third container 22 labeled plasma reduced platelets (CP) is selectively coupled via the tube 36, a valve V4 and a tube 35 to the outlet port PT2. Both second container 20 and third container 22 may also be suspended by weight scales 67 and 77, respectively.

A bag or container 16 for storing an anticoagulant is in fluid communication with the venous access device/phlebotomy needle 24 via a bacteria filter F2, a tube 32 and the Y-connector 30. The bacteria filter F2 prevents any bacteria in the anticoagulant (ACD) container 16 from entering the system. Containers 16, 18, 20, and 22 are preferably plastic bags made of a blood compatible material. Peristaltic pumps P1, P2 and P3, together with the valves V1, V2, V3, and V4 control the direction and duration of flow through the apparatus 10 in response to signals generated by a line sensor 14, a donor pressure monitor (DPM) M1, a system pressure monitor (SPM) M2 and air detectors D1, D2 and D3. The air detectors D1, D2 and D3 detect the absence or presence of fluid. The pressure monitors M1 and M2 monitor pressure levels within the apparatus 10. The line sensor 14 is an optical sensor and detects the presence of blood components passing through the line sensor 14 from the output port PT2.

In initial operation, the pumps P1 and P3 are energized to prime the tube 28 of the apparatus 10 with the anticoagulant from the container 16. The anticoagulant passes through the filter F2 and Y-connector 30 before reaching the air detector D1. The air detector D1 senses the presence of the anticoagulant at D1 and terminates the anticoagulant priming operation. During the priming operation, the valve V2 is open and sterile air displaced from bowl 12 by the anticoagulant enters the top port PT3 of the air/plasma container 18. The venous access device 24 is then inserted into the donor and the draw step is ready to be commenced.

FIG. 4 is a flowchart depicting a method for collecting blood components (e.g., platelets) from a subject, in accordance with one embodiment of the invention. During draw step 401, whole blood is drawn from the subject, typically at a rate of about 80 ml/min. and mixed with the anticoagulant using the pumps P1 and P3 (referring back to FIGS. 1-3). The pump P3 mixes the anticoagulant from the container 16 with the whole blood drawn from the subject or a bag in which it is pooled. The valve V1 is open, allowing the anticoagulated whole blood to pass through the tube 28 and blood filter F1 before being pumped into the separation device 12 through the inlet port PT1.

The whole blood is introduced into the bottom of the separation device 12 through a feed tube (not shown), step 202 of FIG. 4. The ratio of the anticoagulant to whole blood is typically about 1:10. The operation of each of the pumps and valves in the apheresis apparatus 10 can be performed in accordance with desired protocols under the control of a controller (not shown), which may be, for example, a microprocessor.

Referring to FIG. 3, the centrifuge 11 has the fixed inlet port PT1 and the fixed outlet port PT2. The rotary seal 74 fluidly couples the stationary inlet port PT1 to the lower interior portion of the bowl 12, and the outlet port PT2 to an upper portion of the bowl interior for collecting separated fractions. A core 72 occupies a volume coaxial with the interior of bowl 12 and provides a separation region between the wall of the core 72 and the outer bowl wall 70.

As the bowl 12 is rotated, centrifugal forces separate the anticoagulated whole blood admitted into the bottom of the bowl into red blood cells (RBC), white blood cells (WBC), platelets and plasma. The number of rotations of the bowl 12 can be selected, for example, within a range of 4,000 to 6,000 rpm, and is typically 4,800 rpm. The blood is separated into different fractions in accordance with the component densities. The higher density component, i.e., RBC 60, is forced to the outer wall 70 of the bowl 12 while the lower density plasma 66 lies nearer the core 72. A buffy coat 61 is formed between the plasma 66 and the RBC 60. The buffy coat 61 is made up of an inner layer of platelets 64, a transitional layer 68 of platelets and WBC and an outer layer of WBC 62. The plasma 66 is the component closest to the outlet port from the separation region and is the first fluid component displaced from the bowl 12 via the outlet port PT2 as additional anticoagulated whole blood enters the bowl 12 through the inlet port PT1.

Returning to FIG. 1, the displaced plasma passes through the line sensor 14, the tube 36, a 3-way T-connector 26, and the valve V2 (in the open position) and enters the first container 18. The plasma entering the first container 18 is drawn from the container 18 by the pump P2 via tube 42, valve 5 (in the open position), Y-connector 92 and tube 40 from the lower port PT4 and is recirculated into the bowl 12 through the inlet port PT1 via Y-connector 91 and line 41. The recirculated plasma dilutes the anticoagulated whole blood entering the bowl 12 and allows the blood components to separate more readily. An optical sensor 21 is applied to a shoulder portion of the bowl 12 for monitoring each layer of the blood components as they gradually and coaxially advance toward the core 72 from the outer wall 70 of the bowl 12. The optical sensor 21 may be mounted in a position at which it can detect the buffy coat reaching a particular radius, and the steps of drawing the whole blood from the donor 401 and introducing the whole blood into the bowl 402 may be terminated in response to the detection.

The amount of whole blood processed by the bowl 12 may be varied in response to at least one characteristic associated with the whole blood, such as the hematocrit value, the number of platelets, the total amount of blood or the like of the whole blood, as described in copending U.S. patent application Ser. No. 09/392,880, filed Sep. 9, 1999, entitled Apheresis Apparatus and Method for Producing Blood Products, which is hereby incorporated by reference. This variable control can be implemented under the control of a microcomputer, as aforementioned. Alternatively, each of them can be implemented manually.

The platelets are extracted from the bowl into a container, step 403 of FIG. 4. In extracting the platelets from the bowl, various methodologies may be employed, including, without limitation, dwell, surge, and/or push methodologies. For illustrative purposes, platelet extraction based on a dwell and surge technique will now be described in detail.

After the whole blood has been introduced into the centrifuge 11, step 402 of FIG. 4, the valve V1 is closed and the pump P1 is stopped so that blood is no longer drawn from the donor, and dwell is commenced. During the dwell, the pump P2 recirculates plasma 66 through the bowl 12 at a moderate rate (for example, about 100 ml/min. in FIG. 4) for about 20 to 30 seconds. At this flow rate, the buffy coat 61 is diluted by the plasma and widens but the platelets do not leave the bowl 12. The dilution of the buffy coat allows the heavier white blood cells to sediment to the outer side of the buffy coat, resulting in a better separation between the lighter platelets layer 64 and the heavier white blood cells layer 62. As a result, the transitional layer 68 is reduced. The dwell period also allows the flow patterns in the bowl 12 to stabilize and allows more time for microbubbles to leave the bowl 12 and be purged.

After dwell, the surge step is commenced. In the surge, the speed of the pump P2 is increased in 5-10 ml/min. increments to recirculate plasma until reaching a platelet surge velocity of about 200-250 ml/min. The platelet surge velocity is the velocity at which platelets can leave the bowl 12 but not red blood cells or white blood cells. The plasma exiting the bowl becomes cloudy with platelets and this cloudiness is detected by the line sensor 14. The line sensor 14 consists of an LED which emits light through blood components leaving the bowl 12 and a photo detector which receives the light after it passes through the components. The amount of light received by the photo detector is correlated to the density of the fluid passing through the line.

When platelets first start leaving the bowl 12, the line sensor output starts to decrease. The valve V3 is opened and the valve V2 is closed and the platelets are collected in container 20. Once the majority of the platelets are removed from the bowl 12, the fluid exiting the bowl becomes less cloudy. This lessening of cloudiness is detected by the line sensor 14, whereupon valve V3 is closed.

After the platelets have been collected, return step 404 (see FIG. 4) is initiated. During return step 404, the rotation of the bowl 12 is stopped and the remaining blood components in the bowl 12 are returned to the donor by reversal of rotation of the pump P1 via the venous access device 24 with the valve V1 open. The valve V2 is also opened to allow air to enter the centrifuge bowl during the return. The plasma from the container 18 dilutes the remaining blood components in the bowl 12. Namely, the pump P2 mixes the plasma with the returning components in the bowl 12 with the valve V2 open, diluting the returning red blood cells component with plasma to speed up the return time. When the remaining blood components in the bowl have been returned to the donor, the return step 404 is terminated.

Referring to FIG. 4, the steps of drawing whole blood from the donor, step 401, introducing the whole blood into a separation chamber, step 402, extracting platelets from the separation chamber into a container, step 403, and returning the remaining components back to the donor, step 404, are repeated until a desired volume of platelets is sequestered in the container 20, step 405. Typically, steps 401-404 are repeated two to four times, with about 450-500 ml of whole blood processed per cycle. The sequestered platelet concentration is typically about 1.5×10⁶/μL.

The platelets in container 20 are then re-introduced into the bowl 12, step 406 of FIG. 4, forming a layer of platelets that is several times larger than that obtained by processing only one cycle of whole anticoagulated blood. For example, in some embodiments, the platelet layer volume is approximately equal to the average volume of one cycle multiplied by the number of platelet sequestering cycles plus one. The platelets are drawn from port PT5 of container 20 by pump P2 via tube 43, valve 7 (in the open position) Y-connector 92, and tube 40, and input into bowl 12 through the inlet port PT1 via Y-connector 91 and line 41. To minimize contact between the platelets and bowl 12, the bowl 12 may be partly filled with anticoagulated whole blood drawn from the donor 401 prior to re-introduction of the platelets. The whole blood forms a cell bed at the periphery of the bowl 12 that serves as a buffer between the periphery of the bowl and the platelets, reducing platelet clumping. Additionally or alternatively, whole anticoagulated blood may be added to the separation chamber during platelet reintroduction so as to bring platelet layer towards the elutriation radius, or after platelet reintroduction for perfecting platelet separation and standardizing conditions of initiating platelet extraction.

Using, for example, surge or push methodologies, a plasma reduced platelet concentration is extracted from the layer of platelets that now reside in bowl 12, step 407 of FIG. 4. The plasma reduced platelet product is sequestered in container 22 via line sensor 14, tube 36, 3-way T-connector 26 and valve V4 (in the open position). Platelet product concentration is typically in the range of 2.6×10⁶/μL to 5.2×10⁶/μL, which is 2-3 times that of platelets sequestered when processing only one cycle of whole anticoagulated blood.

It should be noted that the surge elutriation technique may use a variety of fluids other than plasma to extract either the platelets or the reduced plasma platelet product from the separation chamber (e.g., saline solution may be used). Additionally, the platelets that are reintroduced into the separation chamber may be re-anticoagulated to prevent the platelets from coagulating and/or clumping. For example, the platelet collection bag 20 or the reduced plasma platelet product bag 22 may be pre-loaded with a quantity of anticoagulant so that the platelets and/or reduced plasma platelet product mix with the anticoagulant as they are drawn from the separation chamber. Additionally or alternatively, sufficient anticoagulant may be added as the whole blood is withdrawn from the subject such that enough anticoagulant is still present in the platelets prior to re-processing. In either scenario, the amount of anticoagulant added the whole blood and/or extracted platelets must be weighed against the safety of the subject. In particular, the amount of anticoagulant should be limited so as to prevent a large quantity of anticoagulant being returned to the subject.

It should also be noted that once the platelets and the reduced plasma platelet product are collected, a platelet preservative solution may be added to help preserve and store the platelets for later use. The preservative solution can be added to the platelets and platelet product after collection (e.g., from a separate bag or storage container), or the platelet collection bag 20 and the reduced plasma platelet product bag 22 may be pre-loaded with the additive solution.

If additional reduced plasma platelet product is required, each of the steps 401-407 may now be repeated until a desired quantity of plasma reduced platelet product is collected. In various embodiments, plasma may be added to the plasma reduced platelet product so as to adjust the plasma reduced product to a predetermined volume or concentration.

As shown in FIG. 5, embodiments of the apheresis apparatus can be three-line systems 500 having, in addition to some or all of the components discussed above with regard to the two line system 10, a dedicated return line 27 and a dedicated draw line 29. In such embodiments, both the return line 27 and the draw line 29 may have a dedicated pump that controls the flow and pressure within the lines. For example, the return line 27 may have a dedicated return pump P5 and the draw line may have a dedicated draw pump P4. In addition to the dedicated pumps, each line may also include a pressure sensor (e.g., pressure sensor M1 on the return line and pressure sensor M2 on the draw line) that allow the system 500 to monitor the pressure within the lines and adjust the flow rate based on the pressure measurements.

Additionally, as mentioned above, platelet additive solution may be added to the collected and stored platelets. To facilitate this process, the two-line system 10 and/or the three-line system 500 may include a platelet additive storage container 510 and a platelet additive line 520 that may be fluidly connected to tube 40 at point 530. In a similar manner to the other lines and tubes within the system, the platelet additive line 520 may also include a valve V7 that prevents/allows flow through the platelet additive line 520. Such embodiments may also have a line 540 fluidly connecting the platelet collection bag 20 and the reduced plasma platelet product bag 22. This line may include a valve V4 and a filter 550, such as a leukoreduction filter.

The described embodiments of the invention are intended to be merely exemplary and numerous variations and modifications will be apparent to those skilled in the art. All such variations and modifications are intended to be within the scope of the present invention. 

We claim:
 1. A system for collecting plasma reduced platelets from a donor, the system comprising: a) means for drawing whole blood from the donor therethrough; b) means for introducing anticoagulant into the whole drawn blood c) a separation chamber for separating the anticoagulated whole blood into a plurality of components, the components including a platelet component, d) a plasma container for storing an anticoagulated plasma component e) a platelet container for storing the platelet component; f) means for returning at least one of the plurality of components from the bowl back to the to the donor; g) a flow means for connecting the means for drawing whole blood, the separation chamber, the plasma container, the platelet container, and the means for returning at least one of the plurality of components; and h) a controller for controlling the flow means, means for returning, and the separation chamber so as to repeatedly draw whole blood from the donor into the separation chamber, extract platelets from the separation chamber into the platelet container, and return remaining components in the separation chamber back to the donor, wherein after a predetermined volume of platelets has been sequestered in the platelet container, platelets from the platelet container are reintroduced into the separation chamber so as to extract a plasma reduced platelet product from the separation chamber.
 2. The system according to claim 1, wherein the separation chamber separates platelets from the whole blood by elutriation with at least one of anticoagulated plasma or synthetic solution.
 3. The system according to claim 1, wherein the separation chamber separates platelets from the whole blood by layer push.
 4. A system for collecting plasma reduced platelets from a donor, the system comprising: a) an apheresis system for drawing whole blood from a donor, separating the whole blood into a plurality of components including a platelet component and a non-platelet component using a separation chamber, and storing the platelet component into a container while the non-platelet blood components are returned back to the donor; and b) a controller for controlling the apheresis system such that upon obtaining a predetermined volume of platelets in the container, platelets from the container are reintroduced into the separation chamber so as to extract a plasma reduced platelet product from the separation chamber.
 5. The system according to claim 4, wherein the separation chamber separates platelets from the whole blood by elutriation with at least one of anticoagulated plasma or synthetic solution.
 6. The system according to claim 4, wherein the separation chamber separates platelets from the whole blood by layer push.
 7. A system for plasma reduced blood component collection during blood processing comprising: a venous access device for drawing a first volume of whole blood from a subject and returning blood components to the subject; a blood component separation device for separating the drawn blood into a first blood component and a second blood component, the blood component separation device configured to send the first blood component to a first blood component bag using a surge elutriation method; a return line fluidly connecting the venous-access device and the blood component separation device for returning the second blood component to the subject; a reintroduction line fluidly connecting the first blood component bag and the blood component separation device, wherein the first blood component within the first blood component bag is reintroduced into the blood component separation device when a second volume of whole blood is withdrawn from the subject, thereby creating an enlarged layer of the first blood component within the blood component separation device, the enlarged layer of first blood component being removed from the blood component separation device using a surge elutriation method.
 8. A system according to claim 7, wherein the blood processing device is a centrifuge bowl.
 9. A system according to claim 7, wherein the first blood component is platelets and the second blood component is red blood cells.
 10. A system according to claim 7, wherein the blood component separation device further separates the whole blood into a third blood component in addition to the first blood component and the second blood component.
 11. A system according to claim 10, wherein the third blood component is plasma.
 12. A system according to claim 10, wherein the plasma is removed from the blood component separation device and stored in a plasma storage container.
 13. A system according to claim 12, wherein the surge elutriation method includes reintroducing the removed plasma into the blood component separation device at an increasing rate until the first blood component is removed from the blood component separation device.
 14. A system according to claim 10, wherein the blood component separation device also returns the third blood component to the subject in addition to the second blood component.
 15. A system according to claim 7 further including an anticoagulant line connected to an anticoagulant source, the anticoagulant line introducing anticoagulant into the drawn blood.
 16. A system according to claim 7, wherein the first blood component from the first and second draw volumes are reintroduced into the blood component separation device when a third volume of whole blood is withdrawn from the subject, thereby creating a second enlarged layer of the first blood component within the blood component separation device, the second enlarged layer of first blood component being removed from the blood component separation device using a surge elutriation method. 